Antibody:	Anti-Gata3 (HG3-31) sc-268
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: otic placode	Present		2A	Expression was detected throughout most of the otic placode.

Structure	Level	Pattern	Image	Note
TS14: otic pit	Present		2B	Expression was detected throughout most of the otic cup.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	2C	Expression was detected only in the otic vesicle. Some cells on the dorsolateral edge may have been unlabeled. No obvious label was observed in the adjacent ectoderm and neural tissue.
TS15: otocyst mesenchyme	Absent		2C	No label was observed in the periotic mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3A	Expression was distributed throughout the dorsolateral and ventral region.
TS17: endolymphatic appendage	Absent		3A

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	3B	Expression was distributed throughout the dorsolateral and ventral region.

Structure	Level	Pattern	Image	Note
TS17: non-neural ectoderm	Present	Regionally restricted	3C	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst	Present	Regionally restricted	3C	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst mesenchyme	Present	Regionally restricted	3C	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.

Structure	Level	Pattern	Image	Note
TS17: non-neural ectoderm	Present	Regionally restricted	3D	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst	Present	Regionally restricted	3D	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst mesenchyme	Present	Regionally restricted	3D	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.

Structure	Level	Pattern	Image	Note
TS17: non-neural ectoderm	Present	Regionally restricted	3I	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst mesenchyme	Present	Regionally restricted	3I	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst	Present	Regionally restricted	3I	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm. Expression was detected in the vestibular atrium and cochlear pouch.

Structure	Level	Pattern	Image	Note
TS17: non-neural ectoderm	Present	Regionally restricted	3J	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst mesenchyme	Present	Regionally restricted	3J	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm.
TS17: otocyst	Present	Regionally restricted	3J	There is continuity between cells labeled in the anterolateral epithelium of the otocyst, the adjacent mesenchyme, and the ectoderm. Expression was detected in the vestibular atrium and cochlear pouch. Expression labeled only one side of the developing cochlear duct, along the posterior/medial edge of the duct, from which the cochlear sensory epithelium develops.
TS17: rhombomere 4	Present		3J

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present		3K
TS17: otocyst	Present	Regionally restricted	3K	Expression was detected in the vestibular atrium and cochlear pouch. Expression labeled only one side of the developing cochlear duct, along the posterior/medial edge of the duct, from which the cochlear sensory epithelium develops.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present		3L
TS17: otocyst	Present	Regionally restricted	3L	Expression was detected in the vestibular atrium and cochlear pouch. Expression labeled only one side of the developing cochlear duct, along the posterior/medial edge of the duct, from which the cochlear sensory epithelium develops.

Structure	Level	Pattern	Image	Note
TS19: otocyst epithelium	Present	Regionally restricted	4A	A narrow band of label was detected in the dorsal epithelium.
TS19: endolymphatic appendage	Absent		4A
TS19: inner ear epithelium	Strong	Regionally restricted	4A	Strong label was detected in the thickened epithelium of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: otocyst epithelium	epithelial cell	Weak	Regionally restricted	4B	Dorsal epithelial cells were weakly stained.
TS19: inner ear mesenchyme		Present	Regionally restricted	4B	Labeled lateral periotic mesenchyme was observed near the dorsal epithelium.
TS19: inner ear		Present	Regionally restricted	4B	Expression was detected in the cochlea.

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Absent		5A

Structure	Level	Pattern	Image	Note
TS19: otocyst	Present	Regionally restricted	5B	Expression was restricted to the dorsal margin of the body of the otocyst.

Structure	Level	Pattern	Image	Note
TS19: otocyst	Present	Regionally restricted	5C	Expression was restricted to the anterior and posterior edges of the dorsal otocyst.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: otocyst		Present	Regionally restricted	5D	Expression was restricted to the anterior and posterior edges of the dorsal otocyst.
TS19: inner ear mesenchyme	mesenchymal cell	Present	Regionally restricted	5D	Expression was detected in the periotic mesenchyme. Expression was close to the anterior semicircular canal.
TS19: inner ear	ectodermal cell	Present	Regionally restricted	5D	Expression was detected close to the anterior semicircular canal.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: inner ear mesenchyme	mesenchymal cell	Present	Regionally restricted	5E	Expression was detected in the periotic mesenchyme. Expression was close to the posterior semicircular canal.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: inner ear mesenchyme		Present	Regionally restricted	5F	Expression was detected in the periotic mesenchyme. Expression was detected in cells likely to form the spiral ganglion.
TS19: inner ear	ectodermal cell	Present	Regionally restricted	5F	Expression was detected close to the anterior semicircular canal.

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present		5G

Structure	Cell Type	Level	Pattern	Image	Note
TS19: inner ear		Present	Regionally restricted	5H	Expression was detected in cells likely to form the spiral ganglion.
TS19: inner ear epithelium	epithelial cell	Present	Regionally restricted	5H	Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
TS19: cranial ganglion		Present	Regionally restricted	5H	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: inner ear epithelium	epithelial cell	Present	Regionally restricted	5I	Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
TS19: cranial ganglion		Present	Regionally restricted	5I	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: inner ear epithelium	epithelial cell	Present	Regionally restricted	5J	Expression was detected in a small population of posterior epithelial cells that lay at the end of the developing cochlea.
TS19: cranial ganglion		Present	Regionally restricted	5J	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5K	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.
TS19: cranial ganglion	Present	Regionally restricted	5K	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5L	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.
TS19: cranial ganglion	Present	Regionally restricted	5L	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5M	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.
TS19: cranial ganglion	Present	Regionally restricted	5M	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5N	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.
TS19: cranial ganglion	Present	Regionally restricted	5N	Expression was detected in a population of cells in the location of the vestibulo-acoustic ganglion on the anterior side of the ventral cochlear duct.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5O	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Present	Regionally restricted	5P	On passing ventrally along the cochlea, expression was detected in the thickened sensory part of the epithelium on the posterior/medial side.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Strong	Regionally restricted	5Q	The extreme tip of the cochlea epithelium was labeled intensely.

Structure	Level	Pattern	Image	Note
TS19: inner ear epithelium	Strong	Regionally restricted	5R	The extreme tip of the cochlea epithelium was labeled intensely.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	6A	Expression was detected in the dorsal edge and medial epithelium adjacent to the endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS20: inner ear mesenchyme	Present		6B
TS20: vestibular ganglion	Present	Regionally restricted	6B	Expression was detected in a small population of cells in the vestibuloacoustic ganglion.
TS20: vestibular component epithelium	Present	Regionally restricted	6B	In a consecutive section, Gata3 staining was complementary to Pax2.

Structure	Level	Pattern	Image	Note
TS20: vestibular component epithelium	Present	Regionally restricted	6C	In a consecutive section, Gata3 staining was complementary to Pax2.
TS20: inner ear mesenchyme	Present	Regionally restricted	6C
TS20: vestibular ganglion	Present	Regionally restricted	6C	Expression was detected in a small population of cells in the vestibuloacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS21: vestibular component epithelium	Weak	Regionally restricted	7B	Weakly labeled areas corresponded to sensory epithelium.
TS21: cochlear duct	Present	Regionally restricted	7B	Expression was detected in the thickened epithelium floor of the cochlear duct.
TS21: cochlear ganglion	Present		7B

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Present	Regionally restricted	7D	Expression was detected in the thickened epithelium floor of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS21: cochlea epithelium	Present	Regionally restricted	8B	Expression was detected in the cochlear epithelium, but only in those regions devoid of nerve fibers.
TS21: cochlear ganglion	Present	Single cells	8B
TS21: inner ear mesenchyme	Present	Single cells	8B

Structure	Level	Pattern	Image	Note
TS23: inner ear mesenchyme	Absent