Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:86070
Grillet N, et al., Dynamic expression of RGS4 in the developing nervous system and regulation by the neural type-specific transcription factor Phox2b. J Neurosci. 2003 Nov 19;23(33):10613-21
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7645429
|
Gene symbol:
|
Gnat3
|
Gene name:
|
G protein subunit alpha transducin 3
|
Probe:
|
pKS-mGust
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
8A:
embryonic day 10.5; Phox2btm1Jbr/Phox2b +
(more )
Note: flat-mount
Specimen
8A:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Phox2btm1Jbr/Phox2b+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
flat-mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: rhombomere 4 |
Present |
|
8A
|
Expressed in facial motoneuronal precursors and their axons, starting in r4 and continues during their migration through r5.
|
TS17: rhombomere 5 |
Present |
|
8A
|
Expressed in facial motoneuronal precursors and their axons, starting in r4 and continues during their migration through r5.
|
Specimen
8B:
embryonic day 11.0; Phox2btm1Jbr/Phox2b +
(more )
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Phox2btm1Jbr/Phox2b+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: rhombomere 4 |
Present |
|
8B
|
Expressed in facial motoneuronal precursors and their axons.
|
Specimen
8B':
embryonic day 11.0; Phox2btm1Jbr/Phox2b +
(more )
Note: Double labeled: blue - Gnat3; brown - Phox2a (assay MGI:7645416).
Specimen
8B':
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Phox2btm1Jbr/Phox2b+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Gnat3; brown - Phox2a (assay MGI:7645416).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: rhombomere 4 |
Present |
|
8B'
|
Coexpressed with Phox2a in facial motoneuronal precursors.
|
Specimen
8C:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Phox2btm1Jbr/Phox2btm1Jbr
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|