Gene Expression Data
Assay Details
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Assay
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Reference:
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J:250407
Blanco MJ, et al., Developmental expression of membrane type 4-matrix metalloproteinase (Mt4-mmp/Mmp17) in the mouse embryo. PLoS One. 2017;12(9):e0184767
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7647200
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Gene symbol:
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Mmp17
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Gene name:
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matrix metallopeptidase 17
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1K:
embryonic day 8.5; Mmp17tm1Ski/Mmp17 +
(more  )
Note: Double labeled: green - Mmp17 reporter; red - Erg (assay MGI:7647168).
Specimen
1K:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Mmp17tm1Ski/Mmp17+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Mmp17 reporter; red - Erg (assay MGI:7647168).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS13: blood vessel |
blood vessel endothelial cell |
Present |
Regionally restricted |
1K
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Co-expression with Erg in endothelial cells.
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Specimen
1L:
embryonic day 8.5; Mmp17tm1Ski/Mmp17 +
(more )
Note: Double labeled: green - Mmp17 reporter; magenta - Pecam1 (assay MGI:7647169).
Specimen
1L:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Mmp17tm1Ski/Mmp17+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Mmp17 reporter; magenta - Pecam1 (assay MGI:7647169).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS13: blood vessel |
blood vessel endothelial cell |
Present |
Regionally restricted |
1L
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Co-expression with Erg in endothelial cells.
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Specimen
3J/L:
embryonic day 10.5; Mmp17tm1Ski/Mmp17 +
(more )
Note: Double labeled: red - Mmp17 reporter; green - Pecam1 (assay MGI:7647169).
Specimen
3J/L:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Mmp17tm1Ski/Mmp17+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mmp17 reporter; green - Pecam1 (assay MGI:7647169).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
aortic endothelial cell |
Present |
Regionally restricted |
3J
,
3L
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Co-expression with Pecam1 in endothelial cells.
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Specimen
S1E:
embryonic day 10.5; Mmp17tm1Ski/Mmp17 +
(more )
Specimen
S1E:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Mmp17tm1Ski/Mmp17+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1H:
embryonic day 10.5; Mmp17tm1Ski/Mmp17 +
(more )
Specimen
S1H:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Mmp17tm1Ski/Mmp17+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools