Probe:	MB18
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Phosphorimaging

Structure	Level	Pattern	Image	Note
TS19: eye	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS20: neural retina	Strong	Regionally restricted	1C/D	Expression was detected in the central region of the neural retina. Within the central retina, the most intense DCC expression was localized to the emerging inner nuclear layer. Weaker expression was seen in patches at the outer edge of the outer nuclear layer. No label was seen in the peripheral regions.

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present	Regionally restricted	2B , 2C , 2D	Bright chains of closely opposed expressing cells were observed extending from the outer surface of the outer nuclear layer through to the expanding inner nuclear layer in the central region of the retina. The domain of high DCC mRNA expression in the inner nuclear layer had expanded towards the periphery in a decreasing gradient. The region of expression was more extensive in the temporal side of the developing retina compared to the nasal region.
TS21: lens	Absent		2B
TS21: pigmented retinal epithelium	Absent		2B
TS21: eyelid	Present		2B

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	3A/B	The intense expression domain above the outer nuclear layer expanded towards the periphery. In the most peripheral regions, chains of strongly expressing cells were situated directly below the region of accumulating postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	3C/D , 3E/F , 3G/H	The intense expression domain above the outer nuclear layer expanded towards the periphery. In the most peripheral regions, chains of strongly expressing cells were situated directly below the region of accumulating postmitotic neurons. Within the E16.5 central retina, where differentiation was more advanced, the positive chains had disappeared.
TS24: retina ganglion cell layer	Present	Regionally restricted	3C/D , 3E/F	Two distinct layers of DCC mRNA expressing E16.5 cells were observed in the central retina. The first in the retinal ganglion cell layer and presumptive amacrine cell layer.
TS24: retina inner nuclear layer	Present	Regionally restricted	3C/D , 3E/F	Two distinct layers of DCC mRNA expressing E16.5 cells were observed in the central retina. The first in the retinal ganglion cell layer and presumptive amacrine cell layer.
TS24: retina outer nuclear layer	Present	Regionally restricted	3E/F , 3C/D	Two distinct layers of DCC mRNA expressing E16.5 cells were observed in the central retina. The second in the outer region of the outer nuclear layer.

Structure	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	Strong		4A/B , 4C/D
TS26: retina inner nuclear layer	Present	Regionally restricted	4C/D , 4A/B	Expression was detected in the amacrine and horizontal cell layers.
TS26: retina outer nuclear layer	Present	Regionally restricted	4C/D , 4A/B	Expression was detected in the amacrine and horizontal cell layers.