Mki67 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:305426 Du G, et al., Proper timing of a quiescence period in precursor prospermatogonia is required for stem cell pool establishment in the male germline. Development. 2021 May 1;148(9):dev194571
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7662955
Gene symbol:	Mki67
Gene name:	antigen identified by monoclonal antibody Ki 67

Antibody:	Anti-Ki67 (ab15580)
Detection system:	Secondary antibody

Results

1_Ki67 E12.5 ©

Specimen 1 E12.5 Ki67: embryonic day 12.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS20: testis	Present		1_Ki67 E12.5	Expression is in proliferating cells.

1_Ki67 E13.5 ©

Specimen 1 E13.5 Ki67: embryonic day 13.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS21: testis	Present		1_Ki67 E13.5	Expression is in proliferating cells.

1_Ki67 E14.5 ©

Specimen 1 E14.5 Ki67: embryonic day 14.4 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS22: testis	Present		1_Ki67 E14.5	Expression is in proliferating cells.

1_Ki67 E16.5 ©

Specimen 1 E16.5 Ki67: embryonic day 16.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS24: testis	Present		1_Ki67 E16.5	Expression is in proliferating cells.

1_Ki67 P1.5 ©

Specimen 1 P1.5 Ki67: postnatal day 1.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS27: testis	Present		1_Ki67 P1.5	Expression is in proliferating cells.

1_Ki67 P6.5 ©

Specimen 1 P6.5 Ki67: postnatal day 6.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		1_Ki67 P6.5	Expression is in proliferating cells.

3A Control E14.5 ©

Specimen 3A Control E14.5: embryonic day 14.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3A Control E14.5

3A Control E16.5 ©

Specimen 3A Control E16.5: embryonic day 16.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present	Single cells	3A Control E16.5

3A Control E18.5 ©

Specimen 3A Control E18.5: embryonic day 18.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present	Single cells	3A Control E18.5

3A Rb1-cKO^Blimp1 E14.5 ©

Specimen 3A Rb1 cKO^Blimp1 E14.5: embryonic day 14.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E14.5	About 85.9% of prospermatogonia were found to be proliferating in this mutant compared to 59.1% of prospermatogonia in controls.

Specimen 3A Rb1-cKO^Blimp1 E16.5: embryonic day 16.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E16.5	About 65% of prospermatogonia were found to be proliferating in this mutant compared to about 2% of prospermatogonia in controls.

Specimen 3A Rb1-cKO^Blimp1 E18.5: embryonic day 18.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E18.5	About 5% of prospermatogonia were found to be proliferating in this mutant which was significantly more than in controls.

Specimen S2A Control: embryonic day 13.5 (more

)
Note: Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS21: testis	Present		S2A Control Ki67 , S2A Control Merge

Specimen S2A Rb1-cKO^Blimp1: embryonic day 13.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: green - Mki67; red - Gcna (assay MGI:7662958).

Structure	Level	Pattern	Image	Note
TS21: testis	Present		S2A Rb1-cKO^Blimp1 Merge , S2A Rb1-cKO^Blimp1 Ki67	Expression in this mutant is similar to controls.

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