Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664447
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1_riboprobe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
3s:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3s:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain ventricular layer |
Present |
Regionally restricted |
3s
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Ventricular zone expression was detected along a transverse line aligned
precisely with the IMB (isthmo-mesencephalic transverse boundary), that is, in between the evaginated Fgf8-positive isthmus and the sulcal midbrain area (both of which lacked Wnt1 signal).
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| TS19: midbrain-hindbrain junction |
Absent |
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3s
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| TS19: midbrain roof plate |
Present |
Regionally restricted |
3s
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Expression extended rostralward along the midbrain roof plate.
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Specimen
3t:
embryonic day 11.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3t:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain ventricular layer |
Present |
Regionally restricted |
3t
|
Ventricular zone expression was detected along a transverse line aligned
precisely with the IMB (isthmo-mesencephalic transverse boundary), that is, in between the evaginated Fgf8-positive isthmus and the sulcal midbrain area (both of which lacked Wnt1 signal).
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| TS19: midbrain-hindbrain junction |
Absent |
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3t
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| TS19: midbrain roof plate |
Present |
Regionally restricted |
3t
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Expression extended rostralward along the midbrain roof plate.
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Specimen
6v:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6v:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain roof plate |
Present |
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6v
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| TS22: midbrain |
Present |
Regionally restricted |
6v
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Expression was detected along the transverse IMB (isthmo-mesencephalic transverse boundary) arch.
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Specimen
6w:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
6w:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain roof plate |
Present |
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6w
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| TS22: midbrain |
Present |
Regionally restricted |
6w
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Expression was detected along the transverse IMB (isthmo-mesencephalic transverse boundary) arch.
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Analysis Tools