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Gene Expression Data
Assay Details
Assay
Reference: J:336866 Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
Assay type: RNA in situ
MGI Accession ID: MGI:7665225
Gene symbol: Zfhx3
Gene name: zinc finger homeobox 3
Probe: Zfhx3_riboprobe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: The authors analyzed data from the Allen Developing Mouse Brain.
Results
Specimen 8y: embryonic day 15.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: midbrain Present Regionally restricted 8y Selective labeling was observed in the basal or tegmental preisthmus.

Specimen 11bb: embryonic day 18.5 (more )
Note: Sample was postfixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS26: midbrain-hindbrain junction Present Regionally restricted 11bb Selectively labeled isthmic superficial masses were observed.
TS26: metencephalon Present Regionally restricted 11bb Positive staining was detected in rhombomere 1 cell masses.
TS26: inferior colliculus Present 11bb
TS26: midbrain Present Regionally restricted 11bb Expression was detected in inferior colliculus/brachium of the inferior colliculus complex. The preisthmus domain was devoid of significant expression.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
09/17/2024
MGI 6.24
The Jackson Laboratory