Antibody:	anti-Nek1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: renal corpuscle	podocyte	Strong		1C 2wk
TS28: renal tubule epithelium	kidney tubule cell	Present		1C 2wk	Expressed in several subtypes of tubular epithelial cells less strongly. Expression was much more prominent in the immature mouse kidney than in the adult kidney.
TS28: renal proximal tubule	epithelial cell of proximal tubule	Present		1C 2wk

Structure	Cell Type	Level	Pattern	Image	Note
TS28: renal corpuscle	podocyte	Strong		1C 8wk	Expression waned as the kidneys aged, was relatively strong in both immature and mature kidneys.
TS28: renal tubule epithelium	kidney tubule cell	Present		1C 8wk	Expressed in several subtypes of tubular epithelial cells less strongly. Expression was much more prominent in the immature mouse kidney than in the adult kidney.
TS28: renal proximal tubule	epithelial cell of proximal tubule	Present		1C 8wk

Structure	Cell Type	Level	Pattern	Image	Note
TS28: renal corpuscle	podocyte	Present		1C 12wk	Expression waned as the kidneys aged, was relatively strong in both immature and mature kidneys.
TS28: renal tubule epithelium	kidney tubule cell	Present		1C 12wk	Expressed in several subtypes of tubular epithelial cells less strongly. Expression was much more prominent in the immature mouse kidney than in the adult kidney.
TS28: renal proximal tubule	epithelial cell of proximal tubule	Present		1C 12wk

Structure	Cell Type	Level	Pattern	Image	Note
TS28: renal corpuscle	podocyte	Present		1C 52wk	Expression waned as the kidneys aged, was relatively strong in both immature and mature kidneys.
TS28: renal tubule epithelium	kidney tubule cell	Present		1C 52wk	Expressed in several subtypes of tubular epithelial cells less strongly. Expression was much more prominent in the immature mouse kidney than in the adult kidney.
TS28: renal proximal tubule	epithelial cell of proximal tubule	Present		1C 52wk

Structure	Cell Type	Level	Pattern	Image	Note
TS28: renal corpuscle	podocyte	Present		1D NEK1	Expressed in the cytoplasm.

Structure	Level	Pattern	Image	Note
TS21: nephrogenic zone	Present		2A , 2B	Relatively strongest in the developmentally primitive nephrogenic zones of the subcapsular cortex.
TS21: renal vesicle	Present		2B , 2A	Most abundantly expressed in epithelial vesicles.

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present	Regionally restricted	2D , 2C	Most abundantly expressed in cells at distinct regions of the lesser curvatures and bases.
TS23: s-shaped body	Present	Regionally restricted	2D , 2C	Most abundantly expressed in cells at distinct regions of the lesser curvatures and bases.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: parietal epithelium of maturing renal corpuscle		Present		2E , 2F	Strongest expression.
TS24: visceral epithelium of maturing renal corpuscle		Present		2E , 2F	Strongest expression.
TS24: renal tubule	kidney tubule cell	Present		2F , 2E	Expressed in the cytoplasm of some tubular epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present	Patchy	6Bc	Patchy upregulation of expression in some tubules but loss of staining in cystically dilated tubules.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present	Patchy	6Bd	Patchy upregulation of expression in some tubules but loss of staining in cystically dilated tubules.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present	Regionally restricted	6Be	Expression was upregulated in some tubules but minimal or absent in other, cystically dilated tubules.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present	Regionally restricted	6Bf	Expression was upregulated in some tubules but minimal or absent in other, cystically dilated tubules.