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Gene Expression Data
Assay Details
Assay
Reference: J:268403 Zhou L, et al., Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development. FASEB J. 2017 Jan;31(1):132-147
Assay type: RT-PCR
MGI Accession ID: MGI:7711846
Gene symbol: Acot1
Gene name: acyl-CoA thioesterase 1
Probe: Acot-pF, Acot-pR
Probe preparation: labelled with SYBR green
Assay notes: The specificity of the primers used was not detailed; both family members have been annotated. Quantitative RT-PCR. Relative expression was normalized to 18s rRNA. The normalized expression level of each gene in the WT E19.5 heart (control) was arbitrarily set as 1.
Results Image: 6A Acot
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
WT E19.5 E19.5 TS26: heart Present 2 µg; total RNA Not Specified Not Specified
S187A E19.5 E19.5 TS26: heart Present 2 µg; total RNA involves: 129S1/Sv Fen1tm5.1Bhsh/Fen1tm5.1BhshNot Specified
WT P1 P1 TS27: heart Present 2 µg; total RNA Not Specified Not Specified
S187A P1 P1 TS27: heart Present 2 µg; total RNA involves: 129S1/Sv Fen1tm5.1Bhsh/Fen1tm5.1BhshNot Specified
Notes:
(a) Differences between WT newborns at P1 and WT embryos at E19.5 revealed that the fatty acid metabolism pathway was up-regulated.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
09/17/2024
MGI 6.24
The Jackson Laboratory