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Cthrc1em1(cre/ERT2)Rph
Endonuclease-mediated Allele Detail
Summary
Symbol: Cthrc1em1(cre/ERT2)Rph
Name: collagen triple helix repeat containing 1; endonuclease-mediated mutation 1, Richard P Harvey
MGI ID: MGI:7712823
Synonyms: Cthrc1-creERT2
Gene: Cthrc1  Location: Chr15:38940327-38950516 bp, + strand  Genetic Position: Chr15, 15.35 cM, cytoband C
Alliance: Cthrc1em1(cre/ERT2)Rph page
Mutation
origin
Strain of Origin:  C57BL/6J
Mutation
description
Allele Type:    Endonuclease-mediated (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Cthrc1em1(cre/ERT2)Rph expression driven by 1 gene
 
Mutation details: sgRNAs (TCTAGGGATCCACGTTGAAAAGG and GCAGGGACTGAAATCGTCAGAGG) were designed insert a cre/ER(T2) fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) immediately before the native STOP codon in the last exon (exon 4) of the collagen triple helix repeat containing 1 (Cthrc1) gene (based on Cthrc1-201 ENSMUST00000067072.5). Additionally, two guanines in the PAM sites were mutated to cytosines in intron 4, downstream of the 3' UTR. (J:101977, J:353336)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Cthrc1 (mouse)
Summary of all recombinase alleles driven by Cthrc1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cthrc1 Mutation:  34 strains or lines available
References
Original:  J:353336 Janbandhu V, et al., Novel Mouse Model for Selective Tagging, Purification, and Manipulation of Cardiac Myofibroblasts. Circulation. 2024 Jun 11;149(24):1931-1934
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory