Tnctm1.1(cre/ERT2)Gcr
Targeted Allele Detail
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| Symbol: |
Tnctm1.1(cre/ERT2)Gcr |
| Name: |
tenascin C; targeted mutation 1, Gerhard Christofori |
| MGI ID: |
MGI:7712998 |
| Gene: |
Tnc Location: Chr4:63878022-63965252 bp, - strand Genetic Position: Chr4, 34.06 cM
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| Alliance: |
Tnctm1.1(cre/ERT2)Gcr page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:101977
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| Parent Cell Line: |
iTL BA1 (ES Cell)
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| Strain of Origin: |
(C57BL/6NTac x 129S6/SvEvTac)F1
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tnctm1.1(cre/ERT2)Gcr expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector was designed to insert a Cre/ERT2 fusion protein into the tenascin C (Tnc) locus. Specifically, this vector includes (from 5' to 3') an FRT-flanked Neomycin-STOP, a cre/ERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand binding domain) and rabbit beta-globin gene polyadenylation signal. The construct was electroporated into (C57BL/6NTac x 129S6/SvEvTac)F1-derived iTL BA1 embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric mice were identified by genotyping and bred to a C57BL/6 FLP mouse line to remove the neomycin cassette.
(J:101977, J:316041)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tnc
(mouse)
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| Original: |
J:316041 Luond F, et al., Distinct contributions of partial and full EMT to breast cancer malignancy. Dev Cell. 2021 Nov 23; |
| All: |
2 reference(s) |
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Analysis Tools
