Ndel1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:176963 Sumigray KD, et al., Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. J Cell Biol. 2011 Aug 22;194(4):631-42
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7713187
Gene symbol:	Ndel1
Gene name:	nudE neurodevelopment protein 1 like 1

Antibody:	anti-Ndel1 (295)
Assay notes:	There were other antibodies mentioned in the paper. It was not specified which one was used.

Results

2L ©

Specimen 2L: embryonic day 17.5 (more

)
Note: Double labeled: red - Ndel1; green - Itgb4 (assay MGI:7713188).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: epidermis basal layer	basal cell of epidermis	Weak	Punctate	2L	Localized to a single punctum, presumably the centrosome. More cortical staining was seen.
TS25: dermis	fibroblast of dermis	Weak	Punctate	2L	Localized to a single punctum, presumably the centrosome.
TS25: epidermis suprabasal layer	suprabasal keratinocyte	Present		2L	Localized to the cell cortex, where desmosomes are abundant.

2M ©

Specimen 2M: embryonic day 17.5; Dsp^tm1Efu/Dsp^tm1Efu, Tg(KRT14-cre)1Efu/0 (more

)
Note: Conditional mutant. Double labeled: red - Ndel1; green - Itgb4 (assay MGI:7713188).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: epidermis suprabasal layer	suprabasal keratinocyte	Present	Diffuse	2M	Localization was specifically disrupted in suprabasal cells, where it was diffuse throughout the cytoplasm.
TS25: epidermis basal layer	basal cell of epidermis	Present	Punctate	2M	Remained associated with the centrosome.
TS25: dermis	fibroblast of dermis	Present	Punctate	2M	Remained associated with the centrosome.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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