Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75859
Wigle JT, et al., An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype. EMBO J. 2002 Apr 2;21(7):1505-13
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7713272
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Gene symbol:
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Prox1
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Gene name:
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prospero homeobox 1
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2A:
embryonic day 12.5; Prox1tm1Gco/Prox1 +
(more  )
Note: Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
2A
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Co-expression with Pecam1 in budding lymphatic endothelial cells.rr
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Specimen
2B:
embryonic day 12.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
2B
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Co-expression with Flt4 in budding lymphatic endothelial cells.
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Specimen
3A:
embryonic day 14.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
Specimen
3A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
3A
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Co-expression with Pecam1 in budding lymphatic endothelial cellss, interspersed with blood vessels and capillaries.
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Specimen
3A':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
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Specimen
3B:
embryonic day 14.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
3B
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Co-expression with Flt4 in budding lymphatic endothelial cells, interspersed with blood vessels and capillaries.
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Specimen
3B':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
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Specimen
6C:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: thoracic body wall |
Present |
Regionally restricted |
6C
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Expression adjacent to cardinal vein.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: thoracic body wall |
Present |
Regionally restricted |
6D
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Expression adjacent to cardinal vein, in fewer cells than in heterozygote.
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Specimen
7A:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1 reporter; red - Lyve1 (assay MGI:7713282).
Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Lyve1 (assay MGI:7713282).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7A
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Co-expression with Lyve1 in lymphatic endothelial cells.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: green - Prox1 reporter; red - Lyve1 (assay MGI:7713282).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7B
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Expression in lymphatic endothelial cells. No co-expression with Lyve1.
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Specimen
7C:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1; red - laminin.
Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Prox1; red - laminin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7C
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Expression in lymphatic endothelial cells.
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Specimen
7D:
embryonic day 11.5; Prox1tm1Gco/ Prox1tm1Gco
(more )
Note: Double labeled: green - Prox1; red - laminin.
Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1; red - laminin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7D
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Expression in lymphatic endothelial cells.
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Specimen
7E:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: green - Prox1 reporter; red - Cd34 (assay MGI:7713285).
Specimen
7E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Cd34 (assay MGI:7713285).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7E
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Expression in lymphatic endothelial cells.
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Prox1 reporter; red - Cd34 (assay MGI:7713285).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7F
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Expression in lymphatic endothelial cells.
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