Antibody:	Anti-EFABP
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	1c , 1b	Expression was detected at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Immunoreactive cells were arranged regularly at short intervals in parallel with the bottom line of the growth plate. These cells were mononuclear and spindle in shape with long processes extending toward the hypertrophic zone of the growth plate. These cells appear to be septoclasts/perivascular cells.
TS28: tibia	perivascular cell	Present	Regionally restricted	1c , 1b	Expression was detected at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Immunoreactive cells were arranged regularly at short intervals in parallel with the bottom line of the growth plate. These cells were mononuclear and spindle in shape with long processes extending toward the hypertrophic zone of the growth plate. These cells appear to be septoclasts/perivascular cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	osteoblast	Absent		2c	Staining did not overlap with alkaline phosphatase-stained osteoblasts of the proximal tibia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	5a	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia did not overlap with Ppara.
TS28: tibia	perivascular cell	Present	Regionally restricted	5a	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia did not overlap with Ppara.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	5b	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia co-localized with Pparb/d.
TS28: tibia	perivascular cell	Present	Regionally restricted	5b	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia co-localized with Pparb/d.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	5c	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia did not overlap with Pparg.
TS28: tibia	perivascular cell	Present	Regionally restricted	5c	Immunoreactivity in septoclasts/perivascular cells in the chondro-osseous junction of the proximal tibia did not overlap with Pparg.

Structure	Level	Pattern	Image	Note
TS24: long bone epiphyseal plate	Present	Widespread	6a	Immunopositive cells were distributed relatively more widely throughout the growth plate at E16 than at postnatal stages. Small immunopositive cells having a single nucleus and some short processes were distributed not only at the bottom of the growth plate (chondro-osseus junction) but also in the metaphysis.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6c	In the growing growth plate of newborn (postnatal week 0) mice, many immunoreactive cells were observed at the chondro-osseus junction. The immunoreactive cells possessed long processes directed toward the bottom of the growth plate cartilage.
TS27: long bone metaphysis		Present	Single cells	6c

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6d	In the tibial growth plate at suckling stages (postnatal week 1), immunopositive cells at the chondro-osseus junction were increased in number; and the distance between the immunoreactive cells was shorter than that in newborns. The number of septoclasts increased in number from E16 to postnatal week 1-2.
TS28: tibia metaphysis		Present	Single cells	6d

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6e	In the tibial growth plate at suckling stages (postnatal week 2), immunopositive cells at the chondro-osseus junction were increased in number; and the distance between the immunoreactive cells was shorter than that in newborns. The number of septoclasts increased in number from E16 to postnatal week 1-2, and the peak number was reached at postnatal week 2.
TS28: tibia metaphysis		Present	Regionally restricted	6e	The number of immunopositive cells in the metaphysis increased from newborn to postnatal week 2.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6f	In the tibial growth plate of young mice after weaning (postnatal week 3), the distribution of immunopositive cells at the chondro-osseus junction was very similar to that at postnatal week 1.
TS28: tibia metaphysis		Present	Single cells	6f	Very few cells were immunopositive in the metaphysis at this time.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6g	In the tibial growth plate of young mice after weaning (postnatal week 4), the distribution of immunopositive cells at the chondro-osseus junction was very similar to that at postnatal week 1.
TS28: tibia metaphysis		Present	Single cells	6g	Very few cells were immunopositive in the metaphysis at this time.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone epiphyseal plate ossification zone	perivascular cell	Present	Regionally restricted	6h	In the growth plate at sub-adult stage (postnatal week 9), numerous immunopositive cells were arranged along the chondro-osseus junction with a distribution pattern similar to that at the postnatal week 3 or 4 stage, but a decrease in number of immunoreactive cells with a distribution at wider distances from one another was clearly observed. The number of septoclasts at postnatal week 9 decreased to about 40% of the number found at postnatal week 2.