Gene Expression Data
Assay Details
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Assay
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Reference:
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J:315095
Bando Y, et al., Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice. J Mol Histol. 2014 Oct;45(5):507-18
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7716430
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Gene symbol:
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Fabp5
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Gene name:
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fatty acid binding protein 5, epidermal
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Antibody:
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Anti-EFABP
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1f:
postnatal week 4
(more  )
Note: Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
Specimen
1f:
(close  )
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Genetic Background:
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DDY
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: long bone epiphyseal plate ossification zone |
perivascular cell |
Present |
Regionally restricted |
1f
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Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with cathepsin B-positive (Ctsb) cells, which identified the cells as septoclasts/perivascular cells.
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| TS28: tibia |
perivascular cell |
Present |
Regionally restricted |
1f
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Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with cathepsin B-positive (Ctsb) cells, which identified the cells as septoclasts/perivascular cells.
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Specimen
1g:
postnatal week 4
(more )
Note: Double labeled: green - Fabp5; red - DAB lectin.
Specimen
1g:
(close )
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Genetic Background:
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DDY
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Fabp5; red - DAB lectin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: long bone epiphyseal plate ossification zone |
Present |
Regionally restricted |
1g
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Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with DBA lectin-positive cells, which identified the cells as septoclasts/perivascular cells.
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| TS28: tibia |
Present |
Regionally restricted |
1g
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Expression at the chondro-osseous junction between the growth plate cartilage and metaphysis of the proximal tibia. Staining overlapped with DBA lectin-positive cells, which identified the cells as septoclasts/perivascular cells.
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Specimen
2b:
postnatal week 4
(more )
Note: Double labeled: green - Fabp5; red - BSA lectin.
Specimen
2b:
(close )
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Genetic Background:
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DDY
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Age:
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postnatal week 4
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Fabp5; red - BSA lectin.
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Specimen
6b:
embryonic day 16.5
(more )
Note: Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
Specimen
6b:
(close )
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Genetic Background:
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DDY
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Applicable
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Fabp5; red - Ctsb (assay MGI:7716431).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: long bone epiphyseal plate |
Present |
Regionally restricted |
6b
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Double immunostaining for Fabp5 and cathepsin B (Ctsb), cells showing co-localization of both proteins were observed in the growth plate.
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Analysis Tools