Vimtm2(flpo)Ggen
Targeted Allele Detail
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| Symbol: |
Vimtm2(flpo)Ggen |
| Name: |
vimentin; targeted mutation 2, Giannicola Genovese |
| MGI ID: |
MGI:7718776 |
| Synonyms: |
VimFLEX(SA-H2B-eGFP-T2a-FlpO-WPRE-pA), VimFlpO |
| Gene: |
Vim Location: Chr2:13579122-13587637 bp, + strand Genetic Position: Chr2, 10.04 cM, cytoband A2
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| Alliance: |
Vimtm2(flpo)Ggen page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:353926
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Allele Type: |
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Targeted (Conditional ready, Recombinase, Reporter) |
| Mutation: |
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Insertion
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Vimtm2(flpo)Ggen expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Vim (MGI:98932) |
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Mutation details: The targeting vector was designed with (from 5'to 3') an inward facing lox2272 site upstream of exon 2 of the vimentin(Vim) gene, followed by exons 3 and 4, and an inverted cassette flanked with inward facing loxP sites. The inverted floxed cassette contains a polyadenylation sequence, a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), a Flp-recombinase (FlpO), T2A sequence, enhanced green fluorescent protein (EGFP), a human HIST1H2BB gene [histone 1 H2bb], a splice acceptor site and a second lox2272 site in opposite orientation to the first, followed by an FRT-flanked neomycin cassette and a second inward facing loxP site all downstream of exon 4.
(J:101977, J:353926)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Vim
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Vim Mutation: |
40 strains or lines available
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| Original: |
J:353926 Perelli L, et al., Evolutionary fingerprints of EMT in pancreatic cancers. bioRxiv. 2023 Sep 19; |
| All: |
2 reference(s) |
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Analysis Tools
