Mcpt8tm2.1(cre/ERT2)Ics
Targeted Allele Detail
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| Symbol: |
Mcpt8tm2.1(cre/ERT2)Ics |
| Name: |
mast cell protease 8; targeted mutation 2.1, Mouse Clinical Institute |
| MGI ID: |
MGI:7863566 |
| Synonyms: |
Mcpt8tm2.1(RFP657/cre/ERT2)Ics |
| Gene: |
Mcpt8 Location: Chr14:56319623-56322730 bp, - strand Genetic Position: Chr14, 28.19 cM
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| Alliance: |
Mcpt8tm2.1(cre/ERT2)Ics page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:82809
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| Parent Cell Line: |
S3 (ES Cell)
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| Strain of Origin: |
C57BL/6NCrl
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Mutation: |
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Insertion
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Mcpt8tm2.1(cre/ERT2)Ics expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A cassette was inserted in exon 1, by homologous recombination in ES cells. An intron was added in exon 1 to stabilize the transgenic cDNA which contains a mutated TagRFP657 far-red monomeric fluorescent protein sequence, an LF2A (2A self-cleaving peptide) sequence which can induce ribosomal skipping during translation, a dual cre-F3-ERT2-F3 cassette which allows expression of creERT2 or cre (if crossed with an flp deleter), and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was removed by breeding with the dre recombinase deleter line C57BL/6N-Gt(ROSA)26Sortm4.1(CAG-dre)Ics (EMMA strain ID EM:15557). TagRFP657 is not detected due to the mutation in its cDNA.
(J:82809)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Mcpt8
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Mcpt8 Mutation: |
26 strains or lines available
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| Original: |
J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013; |
| All: |
1 reference(s) |
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Analysis Tools
