Prg2tm1.1(cre/ERT2)Ics
Targeted Allele Detail
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| Symbol: |
Prg2tm1.1(cre/ERT2)Ics |
| Name: |
proteoglycan 2, bone marrow; targeted mutation 1.1, Mouse Clinical Institute |
| MGI ID: |
MGI:7863603 |
| Synonyms: |
Prg2tm1.1(RFP657/cre/ERT2)Ics |
| Gene: |
Prg2 Location: Chr2:84810805-84813976 bp, + strand Genetic Position: Chr2, 49.45 cM
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| Alliance: |
Prg2tm1.1(cre/ERT2)Ics page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:82809
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| Parent Cell Line: |
S3 (ES Cell)
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| Strain of Origin: |
C57BL/6NCrl
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Prg2tm1.1(cre/ERT2)Ics expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: A 5.6 kb trap cassette was inserted by homologous recombination in ES cells in the intronic region of Prg2 located between exon 2 and exon 3. This cassette contains intron 1 and partial exon sequence of En2 to act as splice donor site, a LF2A (2A self-cleaving peptide) sequence that can induce ribosomal skipping during translation, a mutated TagRFP657 far-red monomeric fluorescent protein sequence, a T2A sequence and a dual cre-F3-ERT2-F3 cassette which allow expression of creERT2 or cre (if crossed with a flp deleter), and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was deleted in vivo by crossing with a C57BL/6N dre ubiquitous deleter. FACS analysis showed efficient and specific creERT2 expression in spleen eosinophils. TagRFP657 is not detected due to the mutation in its cDNA.
(J:82809)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Prg2
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Prg2 Mutation: |
22 strains or lines available
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| Original: |
J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013; |
| All: |
1 reference(s) |
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Analysis Tools
