Gene Expression Data
Assay Details
Assay
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Reference:
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J:39349
Kury P, et al., Protease nexin-1 is expressed at the mouse met-/mesencephalic junction and FGF signaling regulates its promoter activity in primary met-/mesencephalic cells. Development. 1997 Mar;124(6):1251-62
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1335376
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Gene symbol:
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Serpine2
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Gene name:
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serine (or cysteine) peptidase inhibitor, clade E, member 2
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Probe:
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PN-1 probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 9.5
(more )
Specimen
1A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube |
Present |
Regionally restricted |
1A
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PN-1 transcripts were detected in the ventral neural tube at the met-/mesencephalon junction.
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TS15: diencephalon |
Present |
Regionally restricted |
1A
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PN-1 mRNA was also observed in the ventral part of the mes- and diencephalon.
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TS15: future midbrain |
Present |
Regionally restricted |
1A
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PN-1 mRNA was also observed in the ventral part of the mes- and diencephalon.
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TS15: otocyst |
Present |
Regionally restricted |
1A
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PN-1 mRNA was observed at the surface of the otic vesicle.
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TS15: telencephalic vesicle |
Absent |
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1A
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Staining in the telencephalic vesicles was considered as an artefact since it could also be observed in control embryos either hybridized with a sense mouse PN-1 riboprobe or without a riboprobe.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: branchial arch |
Present |
Regionally restricted |
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PN-1 mRNA was also observed in the posterior, dorsal ectoderm of the paws, in the ectoderm of branchial arches and in the ventral ectodermal ridge of the tail.
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TS15: limb |
Present |
Regionally restricted |
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PN-1 mRNA was also observed in the posterior, dorsal ectoderm of the paws, in the ectoderm of branchial arches and in the ventral ectodermal ridge of the tail.
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TS15: tail |
Present |
Regionally restricted |
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PN-1 mRNA was also observed in the posterior, dorsal ectoderm of the paws, in the ectoderm of branchial arches and in the ventral ectodermal ridge of the tail.
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube |
Present |
Regionally restricted |
1B
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A band of neuroepithelial cells surrounding the neural tube at the level of the junction between the met- and mesencephalon was observed.
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TS17: diencephalon |
Present |
Regionally restricted |
1B
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PN-1 mRNA was also observed in the ventral part of the mes- and diencephalon.
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TS17: midbrain |
Present |
Regionally restricted |
1B
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PN-1 mRNA was also observed in the ventral part of the mes- and diencephalon.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
1C
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PN-1 positive neuroepithelial cells were detected in the neural tube at the mes-/metencephalic junction. In situ hybridization of neighboring sections demonstrated a completely overlapping expression pattern for Fgf8.
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Specimen
3F:
embryonic day 11.5
(more )
Specimen
3F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
3F
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PN-1 positive neuroepithelial cells were detected in the neural tube at the mes-/metencephalic junction. In situ hybridization of neighboring sections demonstrated a completely overlapping expression pattern for Fgf8.
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