Gene Expression Data
Assay Details
Assay
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Reference:
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J:23136
Crossley PH, et al., The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Development. 1995 Feb;121(2):439-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1335641
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3C:
embryonic day 6.25
(more )
Specimen
3C:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 6.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5D:
embryonic day 9.5
(more )
Note: Oblique parasagittal section
Specimen
5D:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Oblique parasagittal section
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
5D
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Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
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TS15: future midbrain floor plate |
Present |
Regionally restricted |
5D
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Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
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TS15: future midbrain lateral wall |
Present |
Regionally restricted |
5D
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Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
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TS15: future midbrain roof plate |
Present |
Regionally restricted |
5D
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Expression is in a sharp-narrow band in the region of the midbrain-hindbrain junction (isthmus). This does not include cells of the ventral midline. Expression is immediately caudal to Wnt1 expression.
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TS15: telencephalon floor plate |
Present |
Regionally restricted |
5D
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Expression is in the commisural plate.
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TS15: telencephalon lateral wall |
Present |
Regionally restricted |
5D
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Expression is in the commisural plate.
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TS15: telencephalon roof plate |
Present |
Regionally restricted |
5D
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Expression is in the commisural plate.
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TS15: diencephalon gland |
Present |
Regionally restricted |
5D
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Expression is in the infundibular region of the hypothalamus.
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TS15: future neurohypophysis |
Present |
Regionally restricted |
5D
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Expression is in the posterior pituitary with a sharp caudal boundary at the mammillary area.
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TS15: future forebrain |
Present |
Regionally restricted |
5D
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Expression is in the alar plate of the ventral thalamus and extends lateral to the dorsal midline just rostral to the zona limitans.
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Specimen
5F:
embryonic day 9.5
(more )
Note: Oblique parasagittal section
Specimen
5F:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Oblique parasagittal section
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Structure |
Level |
Pattern |
Image |
Note |
TS15: optic recess |
Present |
Regionally restricted |
5F
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Expression is in a ventral zone.
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TS15: optic stalk |
Present |
Regionally restricted |
5F
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Expression is in a ventral zone.
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Specimen
5H:
embryonic day 12.5
(more )
Note: Midsagittal section
Specimen
5H:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Midsagittal section
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Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
5H
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Expression is at the midbrain-hindbrain junction (isthmus).
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TS20: midbrain |
Present |
Regionally restricted |
5H
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Expression is at the midbrain-hindbrain junction (isthmus).
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TS20: diencephalon roof plate |
Present |
Regionally restricted |
5H
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Expression is rostral to the developing pineal gland.
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TS20: telencephalon |
Present |
Regionally restricted |
5H
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Expression is in the commisural plate.
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TS20: neurohypophysis infundibulum |
Present |
Regionally restricted |
5H
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Expression is in the infundibular ventral midline and posterior pituitary.
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TS20: pituitary gland |
Present |
Regionally restricted |
5H
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Expression is in the infundibular ventral midline and posterior pituitary.
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Specimen
5I:
embryonic day 12.5
(more )
Note: Parasagittal section of the embryo in (I).
Specimen
5I:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Parasagittal section of the embryo in (I).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
5I
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Expression is at the midbrain-hindbrain junction (isthmus).
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TS20: midbrain |
Present |
Regionally restricted |
5I
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Expression is at the midbrain-hindbrain junction (isthmus).
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TS20: optic chiasma |
Present |
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5I
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Expression is also in the optic nerve.
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TS20: optic stalk |
Present |
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5I
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Expression is also in the optic nerve.
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Specimen
7A:
embryonic day 9.25
(more )
Note: This is a stage I limb bud. Transverse section
Specimen
7A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a stage I limb bud. Transverse section
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Specimen
7B:
embryonic day 10.25
(more )
Note: This is a stage II limb bud. Transverse section
Specimen
7B:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 10.25
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a stage II limb bud. Transverse section
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