Assay
|
Reference:
|
J:1380
MacKenzie A, et al., Expression patterns of the homeobox gene, Hox-8, in the mouse embryo suggest a role in specifying tooth initiation and shape. Development. 1992 Jun;115(2):403-20
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Assay type:
|
RNA in situ
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MGI Accession ID:
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MGI:2136262
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Gene symbol:
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Msx2
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Gene name:
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msh homeobox 2
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Probe:
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p-lambda-26
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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E17.0 and E18.0 embryo heads were demineralized in 0.5M EDTA for 4 days, dehydrated through grades of ethanol, and cleared in chloroform prior to embedding in wax.
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|
Results
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
1 B:
embryonic day 10.5-11.0
(more )
Specimen
1 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 10.5-11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2 B:
embryonic day 11.0
(more )
Specimen
2 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3 D:
embryonic day 11.0
(more )
Specimen
3 D:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3 E:
embryonic day 11.0
(more )
Specimen
3 E:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3 F:
embryonic day 11.0
(more )
Specimen
3 F:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4 A,B:
embryonic day 12.0
(more )
Specimen
4 A,B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandibular process |
Present |
Regionally restricted |
4A/B
|
Expression in the dental placode (a small area of epithelial cells a few microns buccal to the thickening dental epithelium) and in the adjacent mesenchyme.
|
TS20: mandibular process mesenchyme |
Present |
Regionally restricted |
4A/B
|
Expression in the dental placode (a small area of epithelial cells a few microns buccal to the thickening dental epithelium) and in the adjacent mesenchyme.
|
TS20: oral epithelium |
Present |
Regionally restricted |
4A/B
|
Expression in the dental placode (a small area of epithelial cells a few microns buccal to the thickening dental epithelium) and in the adjacent mesenchyme.
|
Specimen
5 B:
embryonic day 12.0
(more )
Note: Bud stage of tooth development.
Specimen
5 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Bud stage of tooth development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: mandibular process |
Present |
Regionally restricted |
5B
|
Expression in the dental placode epithelia into the buccal aspect of the external enamel epithelia of the inavginating bud stage tooth germ.
|
TS20: maxillary process |
Present |
Regionally restricted |
5B
|
Expression in the dental placode epithelia into the buccal aspect of the external enamel epithelia of the inavginating bud stage tooth germ.
|
Specimen
6 B:
embryonic day 15.0
(more )
Note: Cap stage of tooth development.
Specimen
6 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Cap stage of tooth development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: lower jaw molar dental papilla |
Present |
Regionally restricted |
6A/B
|
Expression in the dense epithelium of the enamel knot, the immediately adjacent internal enamel epithelium and in sub-adjacent layer of dental papilla mesenchyme cells, the region of the buccal external enamel epithelium derived from the dental placode.
|
TS23: lower jaw molar enamel organ |
Present |
Regionally restricted |
6A/B
|
Expression in the dense epithelium of the enamel knot, the immediately adjacent internal enamel epithelium and in sub-adjacent layer of dental papilla mesenchyme cells, the region of the buccal external enamel epithelium derived from the dental placode.
|
TS23: enamel knot |
Present |
|
6A/B
|
|
TS23: inner enamel epithelium |
Present |
|
6A/B
|
|
Specimen
7 B:
embryonic day 16.0
(more )
Specimen
7 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
7 E:
embryonic day 17.0
(more )
Specimen
7 E:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8 B:
embryonic day 18.0
(more )
Specimen
8 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lower jaw molar dental papilla |
Strong |
Regionally restricted |
8A/B
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Strong expression in the odontoblastic and sub-odontoblastic layers of the dental papilla mesenchyme. Expression is absent from the central and apical regions of the dental papilla mesenchyme.
|
Specimen
10 B:
embryonic day 12.0
(more )
Specimen
10 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: maxillary process epithelium |
Present |
Regionally restricted |
10A/B
|
Expression in the anterior incisor region. Expression in the invaginating dental epithelium (dental lamina, dental placode) and adjacent oral epithelium, particularly on the buccal side (o).
|
TS20: oral epithelium |
Present |
Regionally restricted |
10A/B
|
Expression in the anterior incisor region. Expression in the invaginating dental epithelium (dental lamina, dental placode) and adjacent oral epithelium, particularly on the buccal side (o).
|
Specimen
11 B:
embryonic day 13.5
(more )
Note: Bud stage of tooth development.
Specimen
11 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Bud stage of tooth development.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lower jaw incisor epithelium |
Present |
Regionally restricted |
11A/B
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Expression in the enamel organ, throughout the entire length of the external enamel epithelia on the buccal and cervical surfaces and in the cervical half of the lingual external enamel epithelia.
|
Specimen
12 A:
embryonic day 16.0
(more )
Note: Cap stage of tooth development.
Specimen
12 A:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Cap stage of tooth development.
|
Specimen
13 B:
embryonic day 16.0
(more )
Specimen
13 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
14 B:
embryonic day 18.0
(more )
Note: Bell stage of tooth development.
Specimen
14 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Bell stage of tooth development.
|
Specimen
15 B:
embryonic day 13.0
(more )
Specimen
15 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
16 B:
embryonic day 13.0
(more )
Specimen
16 B:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: vomeronasal organ epithelium |
Present |
Regionally restricted |
16B
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Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
TS21: vomeronasal organ mesenchyme |
Present |
Regionally restricted |
16B
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Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
Specimen
16 E:
embryonic day 15.0
(more )
Specimen
16 E:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: vomeronasal organ epithelium |
Present |
Regionally restricted |
16E
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Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
TS23: vomeronasal organ mesenchyme |
Present |
Regionally restricted |
16E
|
Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
Specimen
16 H:
embryonic day 17.0
(more )
Specimen
16 H:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: vomeronasal organ epithelium |
Present |
Regionally restricted |
16H
|
Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
TS25: vomeronasal organ mesenchyme |
Present |
Regionally restricted |
16H
|
Expression in the thinning epithelia and adjacent mesenchymal condensation on the nasal side of Jacobson's organs.
|
Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
MF1
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|