Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80018
Przemeck GK, et al., Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development. 2003 Jan;130(1):3-13
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2449015
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.5
(more  )
Note: 0-6 somites.
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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Specimen
2B:
embryonic day 8.5
(more )
Note: 0-6 somites.
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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Specimen
Not shown, +/-:
embryonic day 8.5; Dll1tm1Gos/Dll1 +
(more )
Note: 0-6 somites.
Specimen
Not shown, +/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1+
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm1Gos
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
2C
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Expression in this mutant was either left-sided (4/21), or right-sided (4/21), bilateral (3/21), or not detectable (10/21).
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| TS13: primitive streak |
Present |
Regionally restricted |
2C
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Expression around the node was either normal (4/21 left-bias), changed (3/21), or absent (14/21).
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm1Gos
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
2D
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Expression in this mutant was either left-sided (4/21), or right-sided (4/21), bilateral (3/21), or not detectable (10/21).
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| TS13: primitive streak |
Present |
Regionally restricted |
2D
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Expression around the node was either normal (4/21 left-bias), changed (3/21), or absent (14/21).
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1tm1Gos
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
2E
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Expression in this mutant was either left-sided (4/21), or right-sided (4/21), bilateral (3/21), or not detectable (10/21).
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| TS13: primitive streak |
Present |
Regionally restricted |
2E
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Expression around the node was either normal (4/21 left-bias), changed (3/21), or absent (14/21).
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Specimen
Not Shown, +/+:
embryonic day 8.5
(more )
Note: More than six somites.
Specimen
Not Shown, +/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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More than six somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive streak |
Absent |
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Absent to the left and right of the node in embryos with more than 6 somites.
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