Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676609
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Gene symbol:
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Wnt3a
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Gene name:
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wingless-type MMTV integration site family, member 3A
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Probe:
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Wnt3a probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2G:
embryonic day 9.5
(more  )
Note: 21 somite stage.
Specimen
2G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
2G
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Wedge-shaped ventral extension (lateral expansion) of expression in the diencephalon, P2-specific.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
2H
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P2-specific characteristic present in mutants, although size of domain and levels of expression were greatly reduced.
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Specimen
4H:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Present |
Regionally restricted |
4H
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Expression at dorsal midline of diencephalon.
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Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Present |
Regionally restricted |
4I
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Expression at dorsal midline of diencephalon slightly reduced compared with wild type.
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Analysis Tools
