Tnf^tm1Gkl
Targeted Allele Detail

Summary

• Symbol: Tnf^tm1Gkl
• Name: tumor necrosis factor; targeted mutation 1, George Kollias
• MGI ID: MGI:2149024
• Synonyms: TNF-, Tnf0, Tnf-alpha-, TNFalpha^-, TNFalpha KO, TNFKO
• Gene: Tnf Location: Chr17:35418357-35420983 bp, - strand Genetic Position: Chr17, 18.59 cM
• Alliance: Tnf^tm1Gkl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47673
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Replacement of 40 bp of the 5' UTR, all the coding region, including the ATG translation initiation codon, of the first exon and part of the first intron with MC1neopA cassette. Northern blot analysis of LPS stimulated macrophages confirmed disruption of Tnf mRNA expression. (J:47673)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	16 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tnf^tm1Gkl

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Tnf Mutation: 48 strains or lines available

References

• Original: J:47673 Pasparakis M, et al., Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response [see comments]. J Exp Med. 1996 Oct 1;184(4):1397-411
• All: 491 reference(s)