Cd3e^tm1Mal
Targeted Allele Detail

Summary

• Symbol: Cd3e^tm1Mal
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1, Bernard Malissen
• MGI ID: MGI:2155092
• Synonyms: Cd3e^-, Cd3-e^delta5, Cd3-e^delta55, CD3epsilon^-, CD3-epsilon^delta5, CD3epsilon^delta5
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^tm1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29341
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin cassette replaced most of exon 5, which encodes the extracellular domain. Northern analysis detected a faint, aberrant ~1.3kb transcript in thymocytes of homozygous mutant mice. PCR studies of cDNA synthesized from total thymocyte RNA did not detect wild-type mRNA in homozygous mutant mice using an internal, exon 8 oligo probe. However, these studies did detect a faint truncated transcript in homozygous mutant thymocytes, indicating that exon 5 was skipped in splicing events in the mutant allele. Western blot analysis of homozygous mutant thymocytes using a rabbit antiserum to the cytoplasmic carboxy terminal ten amino acids detected a small amount of a truncated protein. (J:29341)

Disease models

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cd3e^tm1Mal

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 54 strains or lines available

References

• Original: J:29341 Malissen M, et al., Altered T cell development in mice with a targeted mutation of the CD3-epsilon gene. EMBO J. 1995 Oct 2;14(19):4641-53
• All: 123 reference(s)