Gsn^tm1Djk
Targeted Allele Detail

Summary

• Symbol: Gsn^tm1Djk
• Name: gelsolin; targeted mutation 1, David J Kwiatkowski
• MGI ID: MGI:2179509
• Gene: Gsn Location: Chr2:35146392-35197904 bp, + strand Genetic Position: Chr2, 23.5 cM
• Alliance: Gsn^tm1Djk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:24346
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into the first coding exon, 60 bp downstream of the initiation codon. Northern blot analysis on RNA derived from various tissues of homozygous mice demonstrated that no transcript was produced from this allele. Activity assays and western blotting experiments confirmed that no functional protein was expressed. (J:24346)

Expression

In Mice Carrying this Mutation:	16 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Gsn Mutation: 46 strains or lines available

References

• Original: J:24346 Witke W, et al., Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin. Cell. 1995 Apr 7;81(1):41-51
• All: 28 reference(s)