Tgm2^tm1Gml
Targeted Allele Detail

Summary

• Symbol: Tgm2^tm1Gml
• Name: transglutaminase 2, C polypeptide; targeted mutation 1, Gerry Melino
• MGI ID: MGI:2180638
• Synonyms: Tg2^-, TG2-, TG2KO, Tgase2-
• Gene: Tgm2 Location: Chr2:157958325-157988312 bp, - strand Genetic Position: Chr2, 78.72 cM
• Alliance: Tgm2^tm1Gml page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67573
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.2 kb region of the gene spanning part of exon 5, intron 5, exon 6, and part of exon 6 was replaced with a neo resistance cassette via homologous recombination thus deleting the active site of exon 6 and results in the loss of protein cross-linking activity. Western blot of liver, thymus, brain, and erythrocyte samples and RT-PCR analysis of thymus from homozygous mutant animals verified the absence of gene expression. However, residual enzyme activity was detected in some tissues which the authors speculate may be attributed to Tgase1. (J:67573)

Disease models

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Tgm2 Mutation: 49 strains or lines available

References

• Original: J:67573 De Laurenzi V, et al., Gene disruption of tissue transglutaminase. Mol Cell Biol. 2001 Jan;21(1):148-55
• All: 50 reference(s)