Krt5^tm1Tmm
Targeted Allele Detail

Summary

• Symbol: Krt5^tm1Tmm
• Name: keratin 5; targeted mutation 1, Thomas M Magin
• MGI ID: MGI:2182612
• Synonyms: K5^-
• Gene: Krt5 Location: Chr15:101615505-101621333 bp, - strand Genetic Position: Chr15, 56.98 cM
• Alliance: Krt5^tm1Tmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76311
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The promoter and first 2 exons were replaced with an Hprt cassette via homologous recombination. Northern blot of homozygous mutant animals showed complete absence of gene expression. Western blot analysis of skin protein extracts from homozygous mutants showed lack of protein expression. (J:76311)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Krt5 Mutation: 13 strains or lines available

References

• Original: J:76311 Peters B, et al., Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex. Mol Biol Cell. 2001 Jun;12(6):1775-89
• All: 6 reference(s)