Elane^tm1Sds
Targeted Allele Detail

Summary

• Symbol: Elane^tm1Sds
• Name: elastase, neutrophil expressed; targeted mutation 1, Steven D Shapiro
• MGI ID: MGI:2182771
• Synonyms: NE^-
• Gene: Elane Location: Chr10:79722146-79724050 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Elane^tm1Sds page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47420
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette replaced exon 2, which encodes the histidine residue of the catalytic triad. Northern blot analysis of total RNA obtained from bone marrow myeloid cells showed that transcript encoded by the targeted allele was absent. Reprobing of the blot showed that the expression of the closely linked proteinase 3 was unaffected by the targeting event. An absence of elastocytic activity derived from the targeted locus was determined via kappa-elastin zymography. (J:47420)

Tumor Data

List all tumor models in MMHCdb carrying Elane^tm1Sds

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Elane Mutation: 25 strains or lines available

References

• Original: J:47420 Belaaouaj A, et al., Mice lacking neutrophil elastase reveal impaired host defense against gram negative bacterial sepsis. Nat Med. 1998 May;4(5):615-8
• All: 71 reference(s)