Tecta^tm1Gpr
Targeted Allele Detail

Summary

• Symbol: Tecta^tm1Gpr
• Name: tectorin alpha; targeted mutation 1, Guy P Richardson
• MGI ID: MGI:2183148
• Synonyms: Tecta^deltaENT
• Gene: Tecta Location: Chr9:42240915-42311225 bp, - strand Genetic Position: Chr9, 23.63 cM
• Alliance: Tecta^tm1Gpr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77634
• Parent Cell Line: CCB (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a neomycin selection cassette disrupted exon 3. RT-PCR and sequence analysis of mutant mice revealed that the insertion of the neo cassette resulted in exon skipping, producing a transcript in which exons 2 and 4 are joined. While, encoded protein was undetected in homozygous mutant mice via Western blot analysis, immunofluorescence analysis showed immunoreactivity in the greater epithelial ridge and in isolated spots in the tectorial membrane. (J:77634)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Tecta Mutation: 133 strains or lines available

References

• Original: J:77634 Legan PK, et al., A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback. Neuron. 2000 Oct;28(1):273-85
• All: 8 reference(s)