Ikbkg^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Ikbkg^tm1Mak
• Name: inhibitor of kappaB kinase gamma; targeted mutation 1, Tak Mak
• MGI ID: MGI:2386267
• Synonyms: NEMO(-), NEMO/Ikkgamma-deficient
• Gene: Ikbkg Location: ChrX:73436896-73497460 bp, + strand Genetic Position: ChrX, 38.0 cM
• Alliance: Ikbkg^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61666
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 1 and 2 with a PGK-neo cassette via homologous recombination resulting in deletion of the first 131 amino acids and a downstream frameshift. Homozygous mutant embryos were detected by PCR genotype analysis up to day E14.5. Western blot analysis of mouse embryonic fibroblasts derived from E12.5 homozygous mutant embryos showed an absence of protein product, thus confirming inactivation of the gene. (J:61666)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbkg Mutation: 16 strains or lines available

References

• Original: J:61666 Rudolph D, et al., Severe liver degeneration and lack of NF-kappaB activation in NEMO/IKKgamma-deficient mice. Genes Dev. 2000 Apr 1;14(7):854-62
• All: 3 reference(s)