Cd79a^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Cd79a^tm1Cgn
• Name: CD79A antigen (immunoglobulin-associated alpha); targeted mutation 1, University of Cologne
• MGI ID: MGI:2388324
• Synonyms: Igalpha^FF-flox
• Gene: Cd79a Location: Chr7:24596922-24601283 bp, + strand Genetic Position: Chr7, 13.49 cM
• Alliance: Cd79a^tm1Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71100
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A loxP site was introduced into the intron upstream of exon 4. A floxed neo cassette and duplicated exons 4 and 5 carrying tyrosine to phenylalanine substitutions at amino acids 182 and 193 (Y182F, Y193F) of the ITAM motif were inserted downstream of the endogenous exon 5. Transient expression of cre recombinase removed the neo cassette leaving behind loxP sites flanking endogenous exons 4 and 5, followed by mutant exons 4 and 5 carrying the amino acid substitutions. (J:71100)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd79a Mutation: 24 strains or lines available

References

• Original: J:71100 Kraus M, et al., Interference with immunoglobulin (Ig)alpha immunoreceptor tyrosine-based activation motif (ITAM) phosphorylation modulates or blocks B cell development, depending on the availability of an Igbeta cytoplasmic tail. J Exp Med. 2001 Aug 20;194(4):455-69
• All: 1 reference(s)