Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Anton Berns
• MGI ID: MGI:2445311
• Synonyms: ACM-cre, Cre-ER^Tm, Gt(ROSA)26Sor^{tm1(cre/Esr1)Brn}, R26cre-ER^T, R26-CreER^T, R26Cre-ERT, Tx-Cre
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(cre/ERT)Brn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80160
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(cre/ERT)Brn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: The endogenous locus was modified by insertion of a construct containing the rabbit beta-globin 3' splice site followed by the cre/ERT fusion gene, which encodes Cre recombinase joined to the G321R mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen (4-OHT), but not by the natural ER ligand 17-beta-estradiol. The fusion gene is followed by the rabbit beta-globin polyadenylation signal and a PGK-puro (puromycin resistance) cassette. Efficient Cre-mediated recombination in transgenic mice carrying floxed reporter genes was reported to reflect constitutive expression of the Gt(ROSA)26Sor from preimplantation stages onward. (J:80160)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gt(ROSA)26Sor (mouse) Summary of all recombinase alleles driven by Gt(ROSA)26Sor. Comparative matrix view of recombinase activities Alliance mouse Gt(ROSA)26Sor gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:80160 Vooijs M, et al., A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent. EMBO Rep. 2001 Apr;2(4):292-7
• All: 70 reference(s)