Tg(Lck-ADPRT)2Tmo
Transgene Detail

Summary

• Symbol: Tg(Lck-ADPRT)2Tmo
• Name: transgene insertion 2, Tarik Moroy
• MGI ID: MGI:2446676
• Synonyms: lck-PARP DBD #2
• Transgene: Tg(Lck-ADPRT)2Tmo Location: unknown
• Alliance: Tg(Lck-ADPRT)2Tmo page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The Lck promoter was observed to drive expression in the T-cell containing tissues including the thymus, spleen and lymph nodes. The highest levels of expression were observed in thymic tissue. Immunohistochemical analysis of T-cell containing tissues confirmed overexpression of a dominant negative peptide consisting of the DNA binding domain (residues 1 through 376) of the human ADP-ribosyltransferase protein. ADP-ribose polymerization was undetected in thymocytes treated with MNNG, a monofunctional alkylating agent, indicating inhibition of the enzymatic activity of the endogenous protein. (J:80883)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:80883 Beneke R, et al., DNA excision repair and DNA damage-induced apoptosis are linked to Poly(ADP-ribosyl)ation but have different requirements for p53. Mol Cell Biol. 2000 Sep;20(18):6695-703
• All: 2 reference(s)