Tg(Prnp-PG14)1Dah
Transgene Detail

Summary

• Symbol: Tg(Prnp-PG14)1Dah
• Name: transgene insertion 1, David A Harris
• MGI ID: MGI:2447086
• Synonyms: PG14 Prp
• Transgene: Tg(Prnp-PG14)1Dah Location: unknown
• Alliance: Tg(Prnp-PG14)1Dah page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-PG14)1Dah expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: The transgene consists of 12 kb of the mouse Prnp promoter region, which includes intron 1, and a mutant Prnp cDNA containing 9 octapeptide insertions rich in glycine and proline residues. This mutation is found in human prion diseases. The transgene also includes an epitope tag for monoclonal antibody 3F4. Transgene expression was verified by Western blot analysis of brain homogenates from transgenic animals. (J:51891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Five transgenic founder were created. Founder A1 carries 28 copies of the transgene, founder A2 carries 5 copies, founder A3 carries 33 copies, founder B carries 2 copies, and founder C carries 4 copies. Founder A1 was unable to breed.

References

• Original: J:51891 Chiesa R, et al., Neurological illness in transgenic mice expressing a prion protein with an insertional mutation. Neuron. 1998 Dec;21(6):1339-51
• All: 16 reference(s)