Cd79b^tm2Mnz
Targeted Allele Detail

Summary

• Symbol: Cd79b^tm2Mnz
• Name: CD79B antigen; targeted mutation 2, Michel C Nussenzweig
• MGI ID: MGI:2447117
• Gene: Cd79b Location: Chr11:106202167-106205388 bp, - strand Genetic Position: Chr11, 68.89 cM
• Alliance: Cd79b^tm2Mnz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66840
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector modified by site-specific mutagenesis to include a nonsense mutation at codon 184 was incorporated at the endogenous locus via homologous recombination. Stopping translation at codon 184 results in the deletion of 45 cytoplasmic amino acids, including those that make up the ITAM. Furthermore, the resultant protein resembles Igh-6 in that it contains only 3 charged cytoplasmic anchor amino acids. An intronic floxed neo cassette was included for selection. (J:66840)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd79b Mutation: 36 strains or lines available

References

• Original: J:66840 Reichlin A, et al., B cell development is arrested at the immature B cell stage in mice carrying a mutation in the cytoplasmic domain of immunoglobulin beta. J Exp Med. 2001 Jan 1;193(1):13-23
• All: 1 reference(s)