Thra^tm2Ven
Targeted Allele Detail

Summary

• Symbol: Thra^tm2Ven
• Name: thyroid hormone receptor alpha; targeted mutation 2, Bjorn Vennstrom
• MGI ID: MGI:2654864
• Synonyms: alpha^tm2, TRalpha2 -
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^tm2Ven page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72959
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An SV40 polyadenylation signal followed by a thymidine kinase/neomycin selection cassette was inserted into exon 10 in order to disrupt the alpha2 isoform, while maintaining the integrity of the alpha1 isoform. The last exon of the RevErbAalpha gene that overlaps with Thra exon 10 (TRalpha2-specific) on the opposite strand was unaffected. Northern blot analysis demonstrated that no detectable transcript for the alpha2 isoform was present in adult brain. The mutation leads to concomitant overexpression of alpha1 as a result of the structure of the 3' exons of the gene. (J:72959)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 40 strains or lines available

References

• Original: J:72959 Salto C, et al., Ablation of TRalpha2 and a Concomitant Overexpression of alpha1 Yields a Mixed Hypo- and Hyperthyroid Phenotype in Mice. Mol Endocrinol. 2001 Dec;15(12):2115-28
• All: 6 reference(s)