Gja1^{tm5(cre/ERT)Kwi}
Targeted Allele Detail

Summary

• Symbol: Gja1^{tm5(cre/ERT)Kwi}
• Name: gap junction protein, alpha 1; targeted mutation 5, Klaus Willecke
• MGI ID: MGI:2676327
• Synonyms: Cx43^Cre-ER(T), Gja1^{tm2(cre/ESR1)Kwi}, Gja1^{tm5(cre/ESR1)Kwi}
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^{tm5(cre/ERT)Kwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85065
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Gja1^{tm5(cre/ERT)Kwi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gja1 (MGI:95713)

• Mutation details: The coding region was replaced with a construct encoding a tamoxifen dependent cre recombinase. The cre recombinase coding region was joined to sequence encoding the ligand binding domain of the human estrogen receptor with a glycine to arginine missense mutation at position 521. This substitution enables the domain to bind to tamoxifen while ablating the estradiol binding ability. Tamoxifen treatment permits the translocation of the cre hybrid protein to the nucleus. (J:85065)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gja1 (mouse) Summary of all recombinase alleles driven by Gja1. Alliance mouse Gja1 gene page

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 60 strains or lines available

References

• Original: J:85065 Kretz M, et al., Altered connexin expression and wound healing in the epidermis of connexin-deficient mice. J Cell Sci. 2003 Aug 15;116(Pt 16):3443-52
• All: 18 reference(s)