Prkaa1^tm1Sbj
Targeted Allele Detail

Summary

• Symbol: Prkaa1^tm1Sbj
• Name: protein kinase, AMP-activated, alpha 1 catalytic subunit; targeted mutation 1, Sebastian B Jorgensen
• MGI ID: MGI:3029359
• Synonyms: alpha1AMPK^-, Ampk^-, AMPKalpha1^-, Prkaa1^tm1Vio
• Gene: Prkaa1 Location: Chr15:5173343-5211380 bp, + strand Genetic Position: Chr15, 1.99 cM
• Alliance: Prkaa1^tm1Sbj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87719
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding amino acids 97 through 157, which make up a portion of the catalytic domain, was replaced with an IRES-betageo cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of liver and skeletal muscle extracts prepared from homozygous mutant mice. (J:87719)

Expression

In Mice Carrying this Mutation:	208072 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Prkaa1^tm1Sbj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Prkaa1 Mutation: 45 strains or lines available

References

• Original: J:87719 Jorgensen SB, et al., Knockout of the alpha2 but not alpha1 5'-AMP-activated protein kinase isoform abolishes 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranosidebut not contraction-induced glucose uptake in skeletal muscle. J Biol Chem. 2004 Jan 9;279(2):1070-9
• All: 115 reference(s)