Ghrl^tm1Mws
Targeted Allele Detail

Summary

• Symbol: Ghrl^tm1Mws
• Name: ghrelin; targeted mutation 1, Mark W Sleeman
• MGI ID: MGI:3045041
• Synonyms: ghrl-
• Gene: Ghrl Location: Chr6:113693080-113696841 bp, - strand Genetic Position: Chr6, 52.84 cM, cytoband E3
• Alliance: Ghrl^tm1Mws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90698
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region was precisely replaced with an in-frame lacZ gene and neo cassette via homologous recombination. Northern blot analysis confirmed the absence of RNA expression in homozygous mutants. LacZ expression recapitulates endogenous gene expression. (J:90698)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Ghrl^tm1Mws/Ghrl^tm1Mws	involves: C57BL/6

Phenotypes:

Affected Systems	hm1
show or hide all annotated terms
adipose tissue	√
abnormal adipose tissue amount	√
homeostasis/metabolism	√
decreased respiratory quotient	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ghrl Mutation: 12 strains or lines available

References

• Original: J:90698 Wortley KE, et al., Genetic deletion of ghrelin does not decrease food intake but influences metabolic fuel preference. Proc Natl Acad Sci U S A. 2004 May 25;101(21):8227-32
• All: 25 reference(s)