Tg(TcraTcrb)425Cbn
Transgene Detail

Summary

• Symbol: Tg(TcraTcrb)425Cbn
• Name: transgene insertion 425, Frank Carbone
• MGI ID: MGI:3046083
• Synonyms: OT-2, OT2, OT-II, OTII, OT-II.2, OT-II alphabeta TCR, OTII TCR, TCR-OT-II.2a, TCRTg, TgN(OT-II.2a), TgN(TcrOT-II), Tg(TcraTcrb)425-2Cbn
• Transgene: Tg(TcraTcrb)425Cbn Location: unknown
• Alliance: Tg(TcraTcrb)425Cbn page

Transgene origin

• Strain of Origin: C57BL/6 x B6.C-H2^bm1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraTcrb)425Cbn expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The transgene was generated by co-injection of two constructs created by separate cloning into a T-cell receptor expression vector the PCR-amplified cDNAs encoding mouse alpha-chain and beta-chain T-cell receptor subunits expressed by a CD4⁺ T-cell hybridoma designated Clone 1.1. The reconstituted receptor pairs with the CD4 coreceptor and is specific for chicken ovalbumin residues 323-339 in the context of H2-Ab1 (I-A^b). (J:87876)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

Notes

Transgenic mice express the mouse alpha-chain and beta-chain T-cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous transgenic mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T-cell ratio, and lymph node T-cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. Phenotypes for the two sublines (OT-II.2)425-2 and 426-6 are described as identical. (J:87876)

References

• Original: J:87876 Barnden MJ, et al., Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol. 1998 Feb;76(1):34-40
• All: 1684 reference(s)