Fmn1^tm1.1Zllr
Targeted Allele Detail

Summary

• Symbol: Fmn1^tm1.1Zllr
• Name: formin 1; targeted mutation 1.1, Rolf Zeller
• MGI ID: MGI:3046837
• Synonyms: delCis, Fmn^delta10.24
• Gene: Fmn1 Location: Chr2:113158081-113547112 bp, + strand Genetic Position: Chr2, 57.3 cM, cytoband C1-qter
• Alliance: Fmn1^tm1.1Zllr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91385
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated from Fmn1^tm1Zlr by crossing to Tg(CMV-cre)1Cgn mice. Cre expression causes the excision of 170 kb containing the remaining exons between 9 and 24; the hygro cassette is also deleted. The predicted protein product is similar to that of Fmn1^tm1Zlr in that the translation is out-of-frame after exon 9. In addition, transcription of Grem1 is disrupted owing to the deletion of a Grem1 regulatory region in an Fmn1 intron. (J:91385)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fmn1 Mutation: 76 strains or lines available

Notes

Homozygous mutant mice display limb deformities and a low frequency of renal abnormalities, but not renal agenesis or aberrant lung patterning phenotypes. This mutation is a hypomorphic allele, allelic to Grem1^tm1Azun. Compound heterozygous mutant mice show a fully penetrant "limb deformity" limb phenotype, but not the phenotypes causing neonatal lethality. Transcription was not detected in the limb bud mesenchyme of compound heterozygous mutant embryos (J:91385).

References

• Original: J:91385 Zuniga A, et al., Mouse limb deformity mutations disrupt a global control region within the large regulatory landscape required for Gremlin expression. Genes Dev. 2004 Jul 1;18(13):1553-64
• All: 2 reference(s)