1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}
Transgenic Allele Detail

Summary

• Symbol: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan}
• Name: RIKEN cDNA 1700016L21 gene; transgene insertion 57, Richard A Lang
• MGI ID: MGI:3057163
• Synonyms: 1700016L21Rik^{Tg(Itgax-DTR/EGFP)57Lan}, CD11c-DTR, CD11c.DTR, CD11cDTR, CD11c-DTR-GFP, CD11c-DTR/GFP, CD11cDTR tg, CD11c/DTR Tg, DCKO, DTR-GFP, DTR-GRP, Itgax-DTR, Tg^{.Itgax-DTR/EGFP.57}Lan, Tg(Itgax-DTR/GFP)57Lan
• Gene: 1700016L21Rik Location: Chr1:80423649-80453377 bp, + strand Genetic Position: Chr1, Syntenic
• Alliance: 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan} page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan} expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  monkey	HBEGF

• 1700016L21Rik^{Tg(Itgax-HBEGF/EGFP)57Lan} expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Itgax (MGI:96609)

• Mutation details: The transgene contains a fusion product involving simian diphtheria toxin receptor and green fluorescent protein under the control of the mouse Itgax promoter (CD11c). Two lines were created (line 11 and line 57) with 1 to 2 copies and 20 copies of the transgene, respectively. All CD8+ and CD8- dendritic cells in the spleen fluoresce and are DT sensitive. Immunohistochemical and flow cytometric analyses show that expression of the transgene is specific to splenic dendritic cells. Line 57 inserted into the gene at 80448681-80455738 (Build GRCm38/mm10) resulting in a 7057 bp deletion. Founder line 57 has a copy number of greater than 20. (J:93488, J:276927)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any 1700016L21Rik Mutation: 4 strains or lines available

References

• Original: J:93488 Jung S, et al., In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity. 2002 Aug;17(2):211-20
• All: 372 reference(s)