Tg(Nes-rtTA)306Rvs
Transgene Detail

Summary

• Symbol: Tg(Nes-rtTA)306Rvs
• Name: transgene insertion 306, Steven A Reeves
• MGI ID: MGI:3505574
• Synonyms: tetO^p27Kip
• Transgene: Tg(Nes-rtTA)306Rvs Location: unknown
• Alliance: Tg(Nes-rtTA)306Rvs page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene contains a rat nestin intron II enhancer/promoter, the reverse tetracycline-controlled transactivator protein (rtTA) gene, an IRES sequence, and a Beta-geo gene. (J:69630)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express the rtTA transgene under the control of the rat nestin intron II enhancer/promoter. Expression occurs in the neuroepithelium of the developing nervous system (embryonic days 10-17), in some neuron subsets of the adult mouse, for example, the cerebellum, hippocampal dentate gyrus, subventricular zone, spinal cord), and in adult testes.

When Tg(Nes-rtTA)306Rvs hemizygous mice are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed.

References

• Original: J:69630 Mitsuhashi T, et al., Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc Natl Acad Sci U S A. 2001 May 22;98(11):6435-40
• All: 14 reference(s)