Tg(Krt1-15-EGFP)2Cot
Transgene Detail

Summary

• Symbol: Tg(Krt1-15-EGFP)2Cot
• Name: transgene insertion 2, George Cotsarelis
• MGI ID: MGI:3511761
• Synonyms: K15-EGFP
• Transgene: Tg(Krt1-15-EGFP)2Cot Location: unknown
• Alliance: Tg(Krt1-15-EGFP)2Cot page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP). The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta). (J:94076, J:94082)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This transgenic line is derived from one of eight EGFP-expressing founder mice. Skin of line 3 mice exhibited the strongest fluorescence under ultraviolet illumination, lines 2, 14, 27, 41, 42 and 44 exhibited less fluorescence, and line 13 an intermediate level. Cells from mice of four lines were screened by fluorescence activated cell sorting (FACS); as line 2 high EGFP-expressing cells separated well from other cells in the sample, this line was used for further FACS analyses.

References

• Original: J:94076 Morris RJ, et al., Capturing and profiling adult hair follicle stem cells. Nat Biotechnol. 2004 Apr;22(4):411-7
• All: 16 reference(s)