Polh^tm1Crey
Targeted Allele Detail

Summary

• Symbol: Polh^tm1Crey
• Name: polymerase (DNA directed), eta (RAD 30 related); targeted mutation 1, Claude-Agnes Reynaud
• MGI ID: MGI:3577345
• Synonyms: Polh^-
• Gene: Polh Location: Chr17:46482281-46513567 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband C
• Alliance: Polh^tm1Crey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98037
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: LoxP sites were introduced to flank exon 4, which contains the DE polymerase motifs conserved in all Y family polymerases. Cre expression mediated excision of exon 4 in ES cells, resulting in the direct splicing of exons 3-5. The out-of-frame junction would introduce a stop codon and therefore a truncated protein of 102 amino acids. (J:98037)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Polh Mutation: 38 strains or lines available

References

• Original: J:98037 Delbos F, et al., Contribution of DNA polymerase {eta} to immunoglobulin gene hypermutation in the mouse. J Exp Med. 2005 Apr 18;201(8):1191-6
• All: 4 reference(s)