Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Andras Nagy
• MGI ID: MGI:3583817
• Synonyms: Gt(ROSA)26Sor^tm1Nagy, Gt(ROSA)26Sor^{tm1(rtTA)Nagy}, R26^rtTA, Rosa26^fs-rtTA, ROSA26-rtTA, ROSA26-rtTA-IRES-EGFP, Rosa-eGFP, Rosa-rtTA^LSL, rtTA^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99607
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. (J:80963, J:99607)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:99607 Belteki G, et al., Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res. 2005;33(5):e51
• All: 189 reference(s)