Il12b^tm2.1Lky
Targeted Allele Detail

Summary

• Symbol: Il12b^tm2.1Lky
• Name: interleukin 12b; targeted mutation 2.1, Richard M Locksley
• MGI ID: MGI:3663352
• Synonyms: get40, Il-12/p40 g
• Gene: Il12b Location: Chr11:44290890-44304860 bp, + strand Genetic Position: Chr11, 25.94 cM, cytoband A5-B2
• Alliance: Il12b^tm2.1Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:112745
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an internal ribosomal entry site (IRES)-enhanced green fluorescent protein (EGFP; Clontech) sequence (followed by a bovine growth hormone poly(A) signal and a loxP site flanked neomycin resistance gene cassette) downstream of the stop codon in exon 8 of the endogenous gene. The construct was electroporated into ES cells that contain the Tg(Prm-cre)70Og transgene that effects the removal of the neo cassette through cre-mediated recombination in the male germline. (J:112745)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il12b Mutation: 33 strains or lines available

References

• Original: J:112745 Reinhardt RL, et al., Visualization of IL-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote Th1 differentiation. J Immunol. 2006 Aug 1;177(3):1618-27
• All: 4 reference(s)