Ptcra^tm1(icre)Hjf
Targeted Allele Detail

Summary

• Symbol: Ptcra^tm1(icre)Hjf
• Name: pre T cell antigen receptor alpha; targeted mutation 1, Hans Jorg Fehling
• MGI ID: MGI:3696089
• Synonyms: pTa/iCre, Ptcra^tm1(cre)Hjf
• Gene: Ptcra Location: Chr17:47066589-47074638 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband D-E1
• Alliance: Ptcra^tm1(icre)Hjf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:117041
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Ptcra^tm1(icre)Hjf expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ptcra (MGI:104857)

• Mutation details: A mini-gene encoding a codon-optimized cre, the SV40 polyadenylation signal and an Frt-flanked neo selection cassette was inserted into the first exon of the Ptcra (pTalpha) gene in ES cells. The neo was excised in vitro by transient transfection with flippase. Expression of cre is observed in immature DP thymocytes and in more mature SP cells. (J:117041, J:197923)

Generation of the Ptcra^tm1(icre)Hjf allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ptcra (mouse) Summary of all recombinase alleles driven by Ptcra. Alliance mouse Ptcra gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ptcra Mutation: 16 strains or lines available

References

• Original: J:117041 Luche H, et al., Faithful activation of an extra-bright red fluorescent protein in 'knock-in' Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol. 2007 Jan;37(1):43-53
• All: 11 reference(s)