Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch
• MGI ID: MGI:3702294
• Synonyms: Gt(ROSA)26Sor^{tm1(M2rtTA)Jae}, M2, M2-rtTA, R26-M2rtTA, R26-rtTA, Rosa26-M2rtTA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} page

Col1a1^{tm1(tetO-Tcfap2c)Hsc}/Col1a1⁺ Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺ mice show increased proliferation and induced hepatic steatosis

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98920
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivator protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 30 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

This mutation was created during the generation of mutant ES cell line KH2, which also contains a frt docking site downstream of the endogenous Col1a1 locus on Chr 11 (Col1a1). The two mutations have subsequently been bred apart.

References

• Original: J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
• All: 305 reference(s)